A lot of Beekeepers have colonies at present that have been split or have swarmed. A new Queen takes at least three weeks to come into lay and this is a tense time for the beekeeper and for the bees. Many beginners assume they have no Queen and quite often go and purchase one needlessly from a beefarmer.
If you know that your box has swarmed, you will roughly know when this happened and so will know when you can start to look for eggs and young larvae i.e. three weeks after your Virgin has hatched.
A box that has swarmed or has been split and is raising a virgin, should initially be left well alone. You don't want to confuse a virgin returning from a mating flight. If she gets lost due to your 'fiddling', you will then have a Queenless colony. However once those three weeks have passed you do need to start to look.
If you see eggs and larvae, then all is well and you will then need to assess the brood pattern. However more often than not you will find no eggs or larvae. This does not mean that your colony is Queenless however. This is the moment when you need to carefully inspect the brood frames. Blow or smoke the bees out of the way.
A Queenright colony will be preparing a brood nest for their new Queen and everything is alright if you see an area of cells on a couple of frames that have been cleaned out and polished in readiness for the new Queen to start laying. There will probably be pollen above these cells and stores in the corner of the fames. In other words the bees are organising themselves and it is order that you will see within your hive. But if you do not see this order after three weeks and you can still see nectar chucked randomly into cells, then there may well be a problem and you should give them a frame of eggs from your other hive. If they raise Queen cells, then evidently they were Queenless. If they seal the brood without creating Queen cells, then you may have a virgin or you may have cussed bees who have decided they no longer want a Queen.
If you get into this scenario, and still after several weeks you can find nothing, then your only hope is to try and get hold of a failing old Queen from someone and come and ask me how to introduce her. She will have low pheromone levels and could possibly be accepted by your Queenless colony. Once the old lady starts laying it will then be possible to bump her off and introduce a Queen. A scenario to be avoided if at all possible as it is difficult to bring back a colony from the brink.
Malcolm Wilkie - 23rd May 2019 (Resent from June 2017 and May 2018)